State diagrams of type I collagen for the rational design of biomimetic materials - Laboratoire de Psychologie Sociale : contextes et régulation
Pré-Publication, Document De Travail Année : 2024

State diagrams of type I collagen for the rational design of biomimetic materials

Isabelle Martinier
Léa Trichet
Patrick Davidson
Francisco M Fernandes

Résumé

Ideally, designing tissue engineering grafts and 3D cell culture materials should rely on mimicking the architecture and composition of the extracellular matrix, which is predominantly comprised of type I collagen. However, while collagen molecules are assembled into fibrils by cells in vivo, well-organized fibrils rarely form spontaneously in vitro. Indeed, the physico-chemical conditions for fibrillogenesis are still poorly understood and their influence on the formation and properties of fibrillar biomimetic materials remains elusive. Here, we establish state diagrams for type I collagen over an unprecedented range of concentration and temperature, showing the collagen denaturation limits, the emergence of fibrils in acidic conditions, and a new regime of collagen molecule/fibril coexistence. We also show how the state diagrams can be used to understand the formation of biomimetic materials by classical methods, as illustrated here by collagen freeze-casting. Therefore, these state diagrams will help to optimize the production of collagen-based biomimetic materials.

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Chimie
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hal-04787602 , version 1 (17-11-2024)

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Isabelle Martinier, Sylvain Deville, Gervaise Mosser, Léa Trichet, Patrick Davidson, et al.. State diagrams of type I collagen for the rational design of biomimetic materials. 2024. ⟨hal-04787602⟩
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